5zqf

X-ray diffraction
3.87Å resolution

Crystal structure of human topoisomerase II beta in complex with 5-iodouridine-containing-DNA in space group P3221

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (3 distinct):
DNA topoisomerase 2-beta Chain: A
Molecule details ›
Chain: A
Length: 803 amino acids
Theoretical weight: 91.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02880 (Residues: 450-1206; Coverage: 47%)
Gene name: TOP2B
Sequence domains:
DNA (5'-D(P*AP*GP*CP*CP*GP*AP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.44 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA/RNA (5'-D(P*AP*GP*C)-R(P*(IU))-D(P*CP*GP*GP*C)-R(P*(IU))-3') Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.99 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P3221
Unit cell:
a: 94.99Å b: 94.99Å c: 231.486Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.203 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided