PDBe 5zog

X-ray diffraction
2.3Å resolution

Crystal Structure of R192F hFen1 in complex with DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 38.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39748 (Residues: 1-333; Coverage: 88%)
Gene names: FEN1, RAD2
Sequence domains:
DNA (5'-D(*CP*CP*CP*GP*TP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.03 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.78 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*GP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 4.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 88.33Å b: 244.8Å c: 70.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.231
Expression systems:
  • Escherichia coli
  • Not provided