5zjr

X-ray diffraction
3.03Å resolution

Structure of AbdB/Exd complex bound to a 'Magenta14' DNA sequence

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein abdominal-B Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 10.2 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P09087 (Residues: 369-452; Coverage: 17%)
Gene names: Abd-B, CG10291
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Homeobox protein extradenticle Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.77 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P40427 (Residues: 237-310; Coverage: 20%)
Gene names: CG8933, exd
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*GP*TP*CP*GP*TP*AP*AP*AP*TP*CP*AP*TP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
DNA (5'-D(P*GP*CP*AP*TP*GP*AP*TP*TP*TP*AP*CP*GP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.28 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 77.33Å b: 49.445Å c: 95.092Å
α: 90° β: 109.18° γ: 90°
R-values:
R R work R free
0.27 0.269 0.288
Expression system: Escherichia coli