5yuy

X-ray diffraction
1.74Å resolution

DNA polymerase IV - DNA ternary complex 9

Released:
Source organism: Escherichia coli K-12
Primary publication:
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
OpenAccess logo Nucleic Acids Res. 46 5875-5885 (2018)
PMID: 29850882

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chains: A, F
Molecule details ›
Chains: A, F
Length: 352 amino acids
Theoretical weight: 39.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47155 (Residues: 2-351; Coverage: 100%)
Gene names: JW0221, b0231, dinB, dinP
Sequence domains:
Structure domains:
DTN1 Chains: B, G
Molecule details ›
Chains: B, G
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DTN Chains: C, H
Molecule details ›
Chains: C, H
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 86.46Å b: 57.05Å c: 110.17Å
α: 90° β: 95.03° γ: 90°
R-values:
R R work R free
0.19 0.188 0.215
Expression systems:
  • Escherichia coli
  • Not provided