PDBe 5yo4

X-ray diffraction
1.37Å resolution

Crystal Structure of B562RIL with engineered disulfide bond K27C-A79C

Released:
Source organism: Escherichia coli
Primary publication:
Protein crystal quality oriented disulfide bond engineering.
OpenAccess logo Protein Cell (2017)
PMID: 29039033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 41.766Å b: 51.287Å c: 89.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.212
Expression system: Escherichia coli