PDBe 5yo3

X-ray diffraction
1.7Å resolution

Crystal Structure of B562RIL with engineered disulfide bond V16C-A29C

Released:
Source organism: Escherichia coli
Primary publication:
Protein crystal quality oriented disulfide bond engineering.
OpenAccess logo Protein Cell (2017)
PMID: 29039033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 40.328Å b: 50.126Å c: 94.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.155 0.188
Expression system: Escherichia coli