5y36

Electron Microscopy
5.2Å resolution

Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
complementary DNA strand Chain: C
Molecule details ›
Chain: C
Length: 41 nucleotides
Theoretical weight: 12.7 KDa
Source organism: Mus musculus
Expression system: Not provided
non-complementary DNA strand Chain: D
Molecule details ›
Chain: D
Length: 41 nucleotides
Theoretical weight: 12.55 KDa
Source organism: Mus musculus
Expression system: Not provided
single-guide RNA Chain: B
Molecule details ›
Chain: B
Length: 99 nucleotides
Theoretical weight: 31.89 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.2Å
Relevant EMDB volumes: EMD-8236
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided