› 5y2c

X-ray diffraction
2.45Å resolution

Crystal structure of Ostrinia furnacalis Group II chitinase catalytic domain 2 E2180L mutant in complex with PENTA-N-ACETYLCHITOOCTAOSE (NAG)5

Released:
DOI: 10.2210/pdb5y2c/pdb
PDB entry 5y2c coloured by chain, front view.
PDB entry 5y2c coloured by chain, side view.
PDB entry 5y2c coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects.
Chen W, Qu M, Zhou Y, Yang Q
J Biol Chem 293 2652-2660 (2018)
PMID: 29317504

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Group II chitinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 43.46 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: A0A221ZS22 (Residues: 2056-2438; Coverage: 13%)
Sequence domains: Glycosyl hydrolases family 18

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 72.742Å b: 90.804Å c: 74.625Å
α: 90° β: 116.32° γ: 90°
R-values:
R R work R free
0.162 0.162 0.203
Expression system: Komagataella phaffii GS115

Quick links

Citations

2 review citations

Biotechnological Approaches for Host Plant Resistance to Insect Pests.
Kumari et al. (2022)
1 more

2 mentions without citation

Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects.
Chen et al. (2018)
1 more