5xy9

X-ray diffraction
2.3Å resolution

Structure of the MST4 and 14-3-3 complex

Released:
Source organism: Homo sapiens
Entry authors: Shi ZB, Zhou ZC

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Serine/threonine-protein kinase 26 Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9P289 (Residues: 314-325; Coverage: 3%)
Gene names: MASK, MST4, STK26

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 72.994Å b: 84.734Å c: 111.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided