5xma

X-ray diffraction
3.8Å resolution

Crystal structure of AsfvPolX in complex with DNA enzyme at P43212 space group

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (3 distinct):
Repair DNA polymerase X Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.55 KDa
Source organism: African swine fever virus BA71V
Expression system: Escherichia coli
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains: DNA polymerase beta thumb
Structure domains:
DNA (36-MER) Chain: E
Molecule details ›
Chain: E
Length: 36 nucleotides
Theoretical weight: 10.99 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA/RNA (5'-D(*AP*CP*GP*AP*GP*AP*GP*AP*GP*AP*T)-R(P*G)-D(P*GP*GP*TP*GP*CP*GP*TP*TP*AP*CP*A)-3') Chain: F
Molecule details ›
Chain: F
Length: 23 nucleotides
Theoretical weight: 7.21 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43212
Unit cell:
a: 78.846Å b: 78.846Å c: 241.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.258 0.285
Expression systems:
  • Escherichia coli
  • Not provided