PDBe 5x11

X-ray diffraction
2.65Å resolution

Crystal structure of Bacillus subtilis PadR in complex with operator DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 188 amino acids
Theoretical weight: 21.75 KDa
Source organism: Bacillus subtilis subsp. spizizenii str. W23
Expression system: Escherichia coli
UniProt:
  • Canonical: E0TW95 (Residues: 1-182; Coverage: 100%)
Gene names: BSUW23_04210, padR
Sequence domains:
DNA (28-MER) Chains: I, M
Molecule details ›
Chains: I, M
Length: 28 nucleotides
Theoretical weight: 8.64 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (28-MER) Chains: J, N
Molecule details ›
Chains: J, N
Length: 28 nucleotides
Theoretical weight: 8.57 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 341.807Å b: 61.567Å c: 120.553Å
α: 90° β: 107.94° γ: 90°
R-values:
R R work R free
0.23 0.228 0.268
Expression systems:
  • Escherichia coli
  • Not provided