5x0y

Electron Microscopy
4.69Å resolution

Complex of Snf2-Nucleosome complex with Snf2 bound to SHL2 of the nucleosome

Released:
Primary publication:
Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure.
Nature 544 440-445 (2017)
PMID: 28424519
Related structures: EMD-6700

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 135 amino acids
Theoretical weight: 15.29 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Sequence domains:
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 122 amino acids
Theoretical weight: 13.52 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Transcription regulatory protein SNF2 Chain: O
Molecule details ›
Chain: O
Length: 735 amino acids
Theoretical weight: 85.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P22082 (Residues: 666-1400; Coverage: 43%)
Gene names: GAM1, RIC1, SNF2, SWI2, TYE3, YOR290C
Sequence domains:
DNA (167-MER) Chain: I
Molecule details ›
Chain: I
Length: 167 nucleotides
Theoretical weight: 51.38 KDa
DNA (167-MER) Chain: J
Molecule details ›
Chain: J
Length: 167 nucleotides
Theoretical weight: 51.72 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.69Å
Relevant EMDB volumes: EMD-6700
Expression system: Escherichia coli