› 5wjb

X-ray diffraction
2.9Å resolution

Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7

Released:
DOI: 10.2210/pdb5wjb/pdb
PDB entry 5wjb coloured by chain, front view.
PDB entry 5wjb coloured by chain, side view.
PDB entry 5wjb coloured by chain, top view.
Source organisms:
Primary publication:
Design considerations in coiled-coil fusion constructs for the structural determination of a problematic region of the human cardiac myosin rod.
Andreas MP, Ajay G, Gellings JA, Rayment I
J Struct Biol 200 219-228 (2017)
PMID: 28743637

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid assembly scaffolding protein; Myosin-7 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 137 amino acids
Theoretical weight: 15.91 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P13848 (Residues: 2-48; Coverage: 48%)
  • Canonical: P12883 (Residues: 1733-1797; Coverage: 3%)
Gene names: 7, MYH7, MYHCB
Sequence domains: Phi29 scaffolding protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 26.456Å b: 93.706Å c: 234.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.249 0.3
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot et al. (2021)
2 more