PDBe 5wet

X-ray diffraction
2.64Å resolution

Crystal Structure of H2-Dd with disulfide-linked 6mer peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Surface protein gp120 Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 561 Da
Source organism: Human immunodeficiency virus type 1 (BRU ISOLATE)
Expression system: Not provided
UniProt:
  • Canonical: P03377 (Residues: 316-321; Coverage: 1%)
Gene name: env
H-2 class I histocompatibility antigen, D-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 32.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01900 (Residues: 26-301; Coverage: 81%)
Gene name: H2-D1
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 47.324Å b: 63.171Å c: 91.558Å
α: 90° β: 106.46° γ: 90°
R-values:
R R work R free
0.192 0.19 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided