PDBe 5wby

X-ray diffraction
3.1Å resolution

Crystal structure of mTOR(deltaN)-mLST8-PRAS40(beta-strand) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 1177 amino acids
Theoretical weight: 134.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 1376-2549; Coverage: 46%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 328 amino acids
Theoretical weight: 36.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Proline-rich AKT1 substrate 1 Chains: O, P
Molecule details ›
Chains: O, P
Length: 98 amino acids
Theoretical weight: 10.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96B36 (Residues: 114-207; Coverage: 37%)
Gene names: AKT1S1, PRAS40

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Unit cell:
a: 138.926Å b: 163.044Å c: 206.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.276
Expression systems:
  • Homo sapiens
  • Escherichia coli