PDBe 5wb2

X-ray diffraction
3.5Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
G-protein coupled receptor homolog US28 Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.16 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: P69333 (Residues: 1-310; Coverage: 88%)
Gene name: US28
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Processed fractalkine Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.63 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P78423 (Residues: 34-100; Coverage: 18%)
Gene names: A-152E5.2, CX3CL1, FKN, NTT, SCYD1
nanobody B1 Chain: D
Molecule details ›
Chain: D
Length: 131 amino acids
Theoretical weight: 14.15 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 47.17Å b: 128.94Å c: 127.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.252 0.296
Expression systems:
  • Homo sapiens
  • Trichoplusia ni
  • Escherichia coli