PDBe 5w5c

X-ray diffraction
1.85Å resolution

Crystal structure of the primed SNARE-Complexin-Synaptotagmin-1 C2AB complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Complexin-1 Chain: E
Molecule details ›
Chain: E
Length: 83 amino acids
Theoretical weight: 9.61 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63041 (Residues: 1-83; Coverage: 62%)
Gene name: Cplx1
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 190-256; Coverage: 23%)
Gene names: Sap, Stx1a
Synaptotagmin-1 Chain: F
Molecule details ›
Chain: F
Length: 282 amino acids
Theoretical weight: 32.38 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21707 (Residues: 140-421; Coverage: 67%)
Gene name: Syt1
Sequence domains: C2 domain
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 77 amino acids
Theoretical weight: 9.03 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 7-83; Coverage: 37%)
  • Best match: P60881-2 (Residues: 7-83)
Gene names: Snap, Snap25
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 65 amino acids
Theoretical weight: 7.47 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 141-204; Coverage: 31%)
Gene names: Snap, Snap25
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 40 amino acids
Theoretical weight: 4.7 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 28-66; Coverage: 34%)
Gene names: Syb2, Vamp2

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 85.663Å b: 89.683Å c: 91.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.232
Expression system: Escherichia coli