› 5w0m

X-ray diffraction
2.3Å resolution

Structure of human TUT7 catalytic module (CM) in complex with U5 RNA

Released:
DOI: 10.2210/pdb5w0m/pdb
PDB entry 5w0m coloured by chain, front view.
PDB entry 5w0m coloured by chain, side view.
PDB entry 5w0m coloured by chain, top view.
Source organisms:
Primary publication:
Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.
Faehnle CR, Walleshauser J, Joshua-Tor L
Nat Struct Mol Biol 24 658-665 (2017)
PMID: 28671666

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178452 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Terminal uridylyltransferase 7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 389 amino acids
Theoretical weight: 44.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5VYS8 (Residues: 983-1365; Coverage: 26%)
Gene names: HS2, KIAA1711, TUT7, ZCCHC6
Sequence domains:
U5 single-stranded RNA Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 5 nucleotides
Theoretical weight: 1.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand ZN 2 x ZN
Ligand IOD 4 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P61
Unit cell:
a: 135.472Å b: 135.472Å c: 179.268Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.229
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

11 review citations

Regulation of microRNA biogenesis and its crosstalk with other cellular pathways.
Treiber et al. (2019)
10 more

3 mentions without citation

Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.
Faehnle et al. (2017)
2 more