5vzm

Solution NMR

Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin

Released:
Source organism: Homo sapiens
Primary publication:
Structural Basis for the Interaction of Mutasome Assembly Factor REV1 with Ubiquitin.
J. Mol. Biol. 430 2042-2050 (2018)
PMID: 29778604

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
DNA repair protein REV1 Chain: B
Molecule details ›
Chain: B
Length: 111 amino acids
Theoretical weight: 11.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBZ9 (Residues: 933-1040; Coverage: 9%)
Gene names: REV1, REV1L

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: simulated annealing
Chemical shifts: BMR30300  
Expression system: Escherichia coli BL21(DE3)