5vnk

X-ray diffraction
2.55Å resolution

Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal LL sorting motif

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein transport protein Sec23A Chain: A
Molecule details ›
Chain: A
Length: 764 amino acids
Theoretical weight: 86.18 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15436 (Residues: 1-764; Coverage: 100%)
Gene name: SEC23A
Sequence domains:
Protein transport protein Sec24A Chain: B
Molecule details ›
Chain: B
Length: 748 amino acids
Theoretical weight: 84.34 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O95486 (Residues: 346-1093; Coverage: 68%)
Gene name: SEC24A
Sequence domains:
Vesicle-trafficking protein SEC22b Chain: C
Molecule details ›
Chain: C
Length: 157 amino acids
Theoretical weight: 18.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08547 (Residues: 1-157; Coverage: 73%)
Gene names: Sec22b, Sec22l1
Sequence domains: Regulated-SNARE-like domain
C-terminal LL Chain: D
Molecule details ›
Chain: D
Length: 5 amino acids
Theoretical weight: 532 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 148.18Å b: 97.615Å c: 130.546Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.199 0.196 0.251
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided