PDBe 5vl3

X-ray diffraction
3.1Å resolution

CD22 d1-d3 in complex with therapeutic Fab Epratuzumab

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
B-cell receptor CD22 Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 323 amino acids
Theoretical weight: 36.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20273 (Residues: 22-330; Coverage: 37%)
  • Best match: P20273-5 (Residues: 4-158)
Gene names: CD22, SIGLEC2
Sequence domains:
Epratuzumab Fab Heavy Chain Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 222 amino acids
Theoretical weight: 24.03 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Epratuzumab Fab Light Chain Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 222 amino acids
Theoretical weight: 24.34 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 87.11Å b: 90.22Å c: 136.632Å
α: 70.83° β: 80.81° γ: 80.99°
R-values:
R R work R free
0.281 0.28 0.295
Expression system: Homo sapiens