X-ray diffraction
1.85Å resolution

Crystal structure of human WEE1 kinase domain in complex with RAC-IV-099, a MK1775 analogue

Source organism: Homo sapiens
Entry authors: Zhu J-Y, Schonbrunn E

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Wee1-like protein kinase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P30291 (Residues: 291-575; Coverage: 44%)
Gene name: WEE1
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 50.29Å b: 44.56Å c: 64.98Å
α: 90° β: 101.94° γ: 90°
R R work R free
0.203 0.203 0.247
Expression system: Escherichia coli BL21(DE3)