PDBe 5vai

Electron Microscopy
4.1Å resolution

Cryo-EM structure of the activated Glucagon-like peptide-1 receptor in complex with G protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Uncharacterized protein Chain: R
Molecule details ›
Chain: R
Length: 461 amino acids
Theoretical weight: 52.58 KDa
Source organism: Oryctolagus cuniculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: G1SGD4 (Residues: 24-422; Coverage: 91%)
Gene name: GLP1R
Sequence domains:
Glucagon-like peptide 1(7-37) Chain: P
Molecule details ›
Chain: P
Length: 31 amino acids
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01275 (Residues: 98-128; Coverage: 19%)
Gene name: GCG
Sequence domains: Peptide hormone
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.37 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-8653
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Trichoplusia ni
  • Escherichia coli