5v0b

X-ray diffraction
2.63Å resolution

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (rIX)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Exonuclease 1 Chain: Z
Molecule details ›
Chain: Z
Length: 358 amino acids
Theoretical weight: 40.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UQ84 (Residues: 1-352; Coverage: 42%)
Gene names: EXO1, EXOI, HEX1
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*CP*TP*AP*GP*TP*CP*GP*AP*CP*AP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*GP*AP*CP*TP*AP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P43212
Unit cell:
a: 73.764Å b: 73.764Å c: 182.796Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.207 0.251
Expression systems:
  • Escherichia coli
  • Not provided