PDBe 5upl

X-ray diffraction
3Å resolution

CDC42 binds PAK4 via an extended GTPase-effector inteface - 2 peptide: PAK4FL, CDC42 - UNREFINED

Released:
Source organism: Homo sapiens
Primary publication:
CDC42 binds PAK4 via an extended GTPase-effector interface.
Proc. Natl. Acad. Sci. U.S.A. (2018)
PMID: 29295922

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PAK 4 Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 51.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O96013 (Residues: 2-591; Coverage: 72%)
  • Best match: O96013-2 (Residues: 2-426)
Gene names: KIAA1142, PAK4
Sequence domains:
Cell division control protein 42 homolog Chain: B
Molecule details ›
Chain: B
Length: 185 amino acids
Theoretical weight: 20.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60953 (Residues: 1-177; Coverage: 93%)
Gene name: CDC42

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P63
Unit cell:
a: 141.652Å b: 141.652Å c: 62.093Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.257 0.31
Expression system: Escherichia coli BL21(DE3)