PDBe 5upk

X-ray diffraction
2.4Å resolution

CDC42 binds PAK4 via an extended GTPase-effector interface - 3 peptide: PAK4cat, PAK4-N45, CDC42

Released:
Source organism: Homo sapiens
Primary publication:
CDC42 binds PAK4 via an extended GTPase-effector interface.
Proc. Natl. Acad. Sci. U.S.A. (2018)
PMID: 29295922

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein kinase PAK 4 Chain: A
Molecule details ›
Chain: A
Length: 48 amino acids
Theoretical weight: 5.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O96013 (Residues: 1-45; Coverage: 8%)
Gene names: KIAA1142, PAK4
Serine/threonine-protein kinase PAK 4 Chain: B
Molecule details ›
Chain: B
Length: 346 amino acids
Theoretical weight: 39.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O96013 (Residues: 286-591; Coverage: 52%)
  • Best match: O96013-2 (Residues: 109-426)
Gene names: KIAA1142, PAK4
Sequence domains: Protein kinase domain
Cell division control protein 42 homolog Chain: C
Molecule details ›
Chain: C
Length: 185 amino acids
Theoretical weight: 20.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60953 (Residues: 1-177; Coverage: 93%)
  • Best match: P60953-1 (Residues: 1-177)
Gene name: CDC42

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 58.465Å b: 61.621Å c: 85.791Å
α: 90° β: 91.57° γ: 90°
R-values:
R R work R free
0.22 0.218 0.264
Expression system: Escherichia coli BL21