PDBe 5un5

X-ray diffraction
2.99Å resolution

Frizzled-8 complex with designed surrogate Wnt agonist, crystal form 1

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Frizzled-8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H461 (Residues: 28-150; Coverage: 18%)
Gene name: FZD8
Sequence domains: Fz domain
Designed Wnt agonist B12 Chains: C, D
Molecule details ›
Chains: C, D
Length: 123 amino acids
Theoretical weight: 13.82 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 41.2Å b: 77.51Å c: 81.43Å
α: 90° β: 92.76° γ: 90°
R-values:
R R work R free
0.228 0.225 0.25
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21