PDBe 5ub5

X-ray diffraction
2.09Å resolution

human POGLUT1 in complex with human Notch1 EGF12 S458T mutant and UDP

Released:

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-xylose + [protein]-L-serine = UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein O-glucosyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 42.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NBL1 (Residues: 29-385; Coverage: 97%)
Gene names: C3orf9, CLP46, KTELC1, MDS010, MDSRP, POGLUT1, UNQ490/PRO1006
Neurogenic locus notch homolog protein 1 Chain: B
Molecule details ›
Chain: B
Length: 42 amino acids
Theoretical weight: 4.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46531 (Residues: 452-491; Coverage: 2%)
Gene names: NOTCH1, TAN1
Sequence domains: EGF-like domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER TURBO X-RAY SOURCE
Spacegroup: P212121
Unit cell:
a: 70.951Å b: 74.072Å c: 83.583Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.193
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)