› 5tzp

X-ray diffraction
1.35Å resolution

Crystal structure of FPV039:Bik BH3 complex

Released:
DOI: 10.2210/pdb5tzp/pdb
PDB entry 5tzp coloured by chain, front view.
PDB entry 5tzp coloured by chain, side view.
PDB entry 5tzp coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.
Anasir MI, Caria S, Skinner MA, Kvansakul M
J Biol Chem 292 9010-9021 (2017)
PMID: 28411240

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166136 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator Bcl-2 homolog Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 17.25 KDa
Source organism: Fowlpox virus strain NVSL
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9J5G4 (Residues: 1-143; Coverage: 82%)
Gene name: FPV039
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Bik Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.78 KDa
Source organism: Gallus gallus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand EDO 7 x EDO
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43
Unit cell:
a: 47.848Å b: 47.848Å c: 74.303Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.172
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4
  • Not provided

Quick links

Citations

7 review citations

The Bcl-2 Family in Host-Virus Interactions.
Kvansakul et al. (2017)
6 more

5 mentions without citation

Mastering Death: The Roles of Viral Bcl-2 in dsDNA Viruses.
Suraweera et al. (2024)
4 more