5tva

X-ray diffraction
2.25Å resolution

A. aeolicus BioW with AMP and CoA

Released:

Function and Biology Details

Reaction catalysed:
ATP + 6-carboxyhexanoate + CoA = AMP + diphosphate + 6-carboxyhexanoyl-CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-carboxyhexanoate--CoA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.45 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67575 (Residues: 1-240; Coverage: 100%)
Gene names: aq_1659, bioW
Sequence domains: 6-carboxyhexanoate--CoA ligase

Ligands and Environments


Cofactor: Ligand COA 1 x COA
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 55.66Å b: 68.86Å c: 156.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.245
Expression system: Escherichia coli