› 5tnw

X-ray diffraction
1.4Å resolution

Phospholipase C gamma-1 C-terminal SH2 domain

Released:
DOI: 10.2210/pdb5tnw/pdb
PDB entry 5tnw coloured by chain, front view.
PDB entry 5tnw coloured by chain, side view.
PDB entry 5tnw coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Multimodal Recognition of Diverse Peptides by the C-Terminal SH2 Domain of Phospholipase C-γ1 Protein.
McKercher MA, Guan X, Tan Z, Wuttke DS
Biochemistry 56 2225-2237 (2017)
PMID: 28376302

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08487 (Residues: 663-759; Coverage: 8%)
Gene name: PLCG1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 33.36Å b: 36.65Å c: 38.78Å
α: 90.2° β: 101.89° γ: 97.2°
R-values:
R R work R free
0.188 0.187 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

SH2 Domain Binding: Diverse FLVRs of Partnership.
Jaber Chehayeb et al. (2020)
4 other articles