5til

X-ray diffraction
2.83Å resolution

Murine class I major histocompatibility complex H-2 Db in complex with LCMV-derived GP33 altered peptide V3P and T-cell receptor P14

Released:
Entry authors: Achour A, Sandalova T, Allerbring EB

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-300; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Pre-GP-C Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: Lymphocytic choriomeningitis mammarenavirus
Expression system: Not provided
UniProt:
  • Canonical: Q9QDK7 (Residues: 33-41; Coverage: 2%)
Gene name: GPC
alpha chain of P14 T cell receptor Chain: G
Molecule details ›
Chain: G
Length: 205 amino acids
Theoretical weight: 23.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Beta chain of murine T cell receptor P14 Chains: H, L
Molecule details ›
Chains: H, L
Length: 238 amino acids
Theoretical weight: 26.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Alpha chain of murine P14 T cell receptor Chain: K
Molecule details ›
Chain: K
Length: 160 amino acids
Theoretical weight: 18.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 61.224Å b: 66.698Å c: 523.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.275
Expression systems:
  • Escherichia coli
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided