PDBe 5tbn

Solution NMR

Solution NMR structure of PHF20 PHD domain in complex with a histone H3K4me2 peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PHD finger protein 20 Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BVI0 (Residues: 646-699; Coverage: 5%)
Gene names: C20orf104, GLEA2, HCA58, NZF, PHF20, TZP
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-12; Coverage: 8%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: simulated annealing
Chemical shifts: BMR30177  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided