X-ray diffraction
2.65Å resolution

Structure of epoxyqueuosine reductase from Bacillus subtilis with the Asp134 catalytic loop swung out of the active site.


Function and Biology Details

Reaction catalysed:
Queuosine(34) in tRNA + acceptor + H(2)O = epoxyqueuosine(34) in tRNA + reduced acceptor
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Epoxyqueuosine reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 48.74 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
  • Canonical: P97030 (Residues: 1-386; Coverage: 100%)
Gene names: BSU08910, queG, ygaP, yhbA
Sequence domains:
Molecule details ›
Chain: X
Length: 17 nucleotides
Theoretical weight: 5.43 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 44.763Å b: 111.03Å c: 95.971Å
α: 90° β: 99.13° γ: 90°
R R work R free
0.239 0.238 0.266
Expression systems:
  • Escherichia coli
  • Not provided