PDBe 5t1g

X-ray diffraction
1.9Å resolution

chromo shadow domain of CBX1 in complex with a histone peptide

Released:
Source organism: Homo sapiens
Entry authors: Liu Y, Tempel W, Bountra C, Arrowsmith CH, Edwards AM, Min J, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromobox protein homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83916 (Residues: 108-185; Coverage: 42%)
Gene names: CBX, CBX1
Sequence domains: Chromo shadow domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.87 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 39-53; Coverage: 11%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: F222
Unit cell:
a: 69.657Å b: 76.887Å c: 78.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided