PDBe 5syo

X-ray diffraction
2Å resolution

Crystal structure of a chimeric acetylcholine binding protein from Aplysia californica (Ac-AChBP) containing loop C from the human alpha 3 nicotinic acetylcholine receptor in complex with Cytisine

Released:
Entry authors: Bobango J, Wu J, Talley IT, Talley TT

Function and Biology Details

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal acetylcholine receptor subunit alpha-3; Soluble acetylcholine receptor Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P32297 (Residues: 86-86, 215-230; Coverage: 4%)
  • Canonical: Q8WSF8 (Residues: 18-71, 73-198, 215-236; Coverage: 92%)
  • nullnull
Gene names: CHRNA3, NACHRA3
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 68.596Å b: 78.077Å c: 120.343Å
α: 90° β: 93.34° γ: 90°
R-values:
R R work R free
0.205 0.204 0.227
Expression system: Homo sapiens