PDBe 5sw3

X-ray diffraction
1.38Å resolution

Crystal Structure of TGT in complex with 3-Pyridinecarboxylic acid, 6-(dimethylamino)

Released:
Entry authors: Hassaan E, Heine A, Klebe G

Function and Biology Details

Reaction catalysed:
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.76 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28720 (Residues: 10-384; Coverage: 97%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 89.027Å b: 64.071Å c: 70.453Å
α: 90° β: 92.82° γ: 90°
R-values:
R R work R free
0.135 0.133 0.162
Expression system: Escherichia coli BL21(DE3)