5suq

X-ray diffraction
6Å resolution

Crystal structure of the THO-Sub2 complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent RNA helicase SUB2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 446 amino acids
Theoretical weight: 50.38 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07478 (Residues: 1-446; Coverage: 100%)
Gene names: SUB2, YDL084W
Sequence domains:
Tex1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 400 amino acids
Theoretical weight: 34.06 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
Tho2, Hpr1, Mft1, and Thp2 Chains: M, N
Molecule details ›
Chains: M, N
Length: 2300 amino acids
Theoretical weight: 195.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 153.339Å b: 319.534Å c: 176.444Å
α: 90° β: 100.96° γ: 90°
R-values:
R R work R free
0.436 0.436 0.434
Expression systems:
  • Escherichia coli
  • Trichoplusia ni