5oxv

X-ray diffraction
6.72Å resolution

Structure of the 4_601_157 tetranucleosome (C2 form)

Released:
Source organism: Xenopus laevis
Primary publication:
Capturing structural heterogeneity in chromatin fibers.
J. Mol. Biol. (2017)
PMID: 28893533

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H2B 1.1 Chains: D, H, N, R
Molecule details ›
Chains: D, H, N, R
Length: 126 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 1-126; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H2A type 1 Chains: C, G, M, Q
Molecule details ›
Chains: C, G, M, Q
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Histone H4 Chains: B, F, L, P
Molecule details ›
Chains: B, F, L, P
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H3.2 Chains: A, E, K, O
Molecule details ›
Chains: A, E, K, O
Length: 135 amino acids
Theoretical weight: 15.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
DNA STRAND 1 (601-based sequence model) Chain: J
Molecule details ›
Chain: J
Length: 312 nucleotides
Theoretical weight: 96.89 KDa
DNA STRAND 2 (601-based sequence model) Chain: I
Molecule details ›
Chain: I
Length: 313 nucleotides
Theoretical weight: 96.15 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 348.839Å b: 63.274Å c: 271.361Å
α: 90° β: 124.74° γ: 90°
R-values:
R R work R free
0.321 0.317 0.352
Expression system: Escherichia coli