PDBe 5oqp

X-ray diffraction
2.98Å resolution

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (crystal form I)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Condensin complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 152 amino acids
Theoretical weight: 18.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P38170 (Residues: 384-529; Coverage: 19%)
Gene names: BRN1, YBL0830, YBL097W
Condensin complex subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 869 amino acids
Theoretical weight: 99.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q06680 (Residues: 6-932; Coverage: 84%)
Gene names: YCG1, YCS5, YDR325W
Sequence domains: Nuclear condensing complex subunits, C-term domain
DNA (5'-D(*GP*AP*TP*GP*TP*GP*TP*AP*GP*CP*TP*AP*CP*AP*CP*AP*TP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 nucleotides
Theoretical weight: 5.52 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 80.14Å b: 114.833Å c: 155.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.262
Expression system: Escherichia coli K-12