5ohf

X-ray diffraction
1.8Å resolution

Globin sensor domain of AfGcHK (FeIII form) in complex with cyanide, partially reduced

Released:

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-coupled histidine kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 161 amino acids
Theoretical weight: 18.37 KDa
Source organism: Anaeromyxobacter sp. Fw109-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7HD43 (Residues: 2-161; Coverage: 42%)
Gene names: Anae109_2438, gchK

Ligands and Environments


Cofactor: Ligand HEM 8 x HEM
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P41212
Unit cell:
a: 77.685Å b: 77.685Å c: 441.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.239
Expression system: Escherichia coli BL21(DE3)