PDBe 5oai

X-ray diffraction
2Å resolution

Structure of MDM2 with low molecular weight inhibitor

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 11.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00987 (Residues: 18-113; Coverage: 20%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I222
Unit cell:
a: 49.731Å b: 59.216Å c: 83.897Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.227
Expression system: Escherichia coli