› 5o01

X-ray diffraction
2.5Å resolution

Crystal structure of BtubC (BKLC) from P. vanneervenii

Released:
DOI: 10.2210/pdb5o01/pdb
PDB entry 5o01 coloured by chain, front view.
PDB entry 5o01 coloured by chain, side view.
PDB entry 5o01 coloured by chain, top view.
Source organism: Prosthecobacter vanneervenii
Primary publication:
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.
Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Löwe J
Proc Natl Acad Sci U S A 114 E5950-E5958 (2017)
PMID: 28673988

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial kinesin light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 29.22 KDa
Source organism: Prosthecobacter vanneervenii
Expression system: Escherichia coli
UniProt:
  • Canonical: A8Y5U5 (Residues: 1-256; Coverage: 100%)
Gene name: bklc
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 119.734Å b: 119.734Å c: 239.366Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.237
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Prokaryotic cytoskeletons: protein filaments organizing small cells.
Wagstaff et al. (2018)
4 more

1 mention without citation

Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.
Deng et al. (2017)