5nzs

Electron Microscopy
10.1Å resolution

The structure of the COPI coat leaf in complex with the ArfGAP2 uncoating factor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-129540 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Coatomer subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 1262 amino acids
Theoretical weight: 142.53 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8CIE6 (Residues: 1-1224; Coverage: 100%)
Gene name: Copa
Sequence domains:
Coatomer subunit beta Chain: B
Molecule details ›
Chain: B
Length: 968 amino acids
Theoretical weight: 109.15 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9JIF7 (Residues: 1-953; Coverage: 100%)
Gene names: Copb, Copb1
Sequence domains:
Coatomer subunit beta' Chain: C
Molecule details ›
Chain: C
Length: 905 amino acids
Theoretical weight: 102.57 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O55029 (Residues: 1-905; Coverage: 100%)
Gene name: Copb2
Sequence domains:
Coatomer subunit delta Chain: D
Molecule details ›
Chain: D
Length: 511 amino acids
Theoretical weight: 57.3 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5XJY5 (Residues: 1-511; Coverage: 100%)
Gene names: Arcn1, Copd
Sequence domains:
ADP-ribosylation factor 1 Chains: F, M, R
Molecule details ›
Chains: F, M, R
Length: 181 amino acids
Theoretical weight: 20.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11076 (Residues: 1-181; Coverage: 100%)
Gene names: ARF1, D1244, YDL192W
Sequence domains: ADP-ribosylation factor family
Coatomer subunit gamma-1 Chains: G, K
Molecule details ›
Chains: G, K
Length: 874 amino acids
Theoretical weight: 97.62 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9QZE5 (Residues: 1-874; Coverage: 100%)
Gene names: Copg, Copg1
Sequence domains:
Coatomer subunit zeta-1 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 177 amino acids
Theoretical weight: 20.22 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61924 (Residues: 1-177; Coverage: 100%)
Gene names: Copz, Copz1
Sequence domains: Clathrin adaptor complex small chain
ADP-ribosylation factor GTPase-activating protein 2 Chain: P
Molecule details ›
Chain: P
Length: 520 amino acids
Theoretical weight: 56.63 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3MID3 (Residues: 1-520; Coverage: 100%)
Gene names: Arfgap2, Zfp289, Znf289
Sequence domains: Putative GTPase activating protein for Arf

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 10.1Å
Relevant EMDB volumes: EMD-3721
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli