5nnp

X-ray diffraction
2.6Å resolution

Structure of Naa15/Naa10 bound to HypK-THB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
TPR_REGION domain-containing protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 745 amino acids
Theoretical weight: 84.91 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S4M4 (Residues: 2-744; Coverage: 100%)
Gene name: CTHT_0031530
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
N-acetyltransferase domain-containing protein Chains: B, F
Molecule details ›
Chains: B, F
Length: 195 amino acids
Theoretical weight: 22.44 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SEE8 (Residues: 2-189; Coverage: 77%)
Gene name: CTHT_0063490
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase
HYPK_UBA domain-containing protein Chains: C, G
Molecule details ›
Chains: C, G
Length: 62 amino acids
Theoretical weight: 6.59 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SCY6 (Residues: 83-126; Coverage: 35%)
Gene name: CTHT_0058830
Ser-Glu-Ser-Ser Chains: I, L
Molecule details ›
Chains: I, L
Length: 4 amino acids
Theoretical weight: 408 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 93.319Å b: 106.041Å c: 130.937Å
α: 90° β: 94.98° γ: 90°
R-values:
R R work R free
0.164 0.161 0.22
Expression systems:
  • Escherichia coli
  • Not provided