5nj8

X-ray diffraction
3.3Å resolution

Structural basis for aryl hydrocarbon receptor mediated gene activation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Aryl hydrocarbon receptor Chains: A, C
Molecule details ›
Chains: A, C
Length: 254 amino acids
Theoretical weight: 28.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35869 (Residues: 23-273; Coverage: 30%)
Gene names: AHR, BHLHE76
Sequence domains:
Aryl hydrocarbon receptor nuclear translocator Chains: B, D
Molecule details ›
Chains: B, D
Length: 267 amino acids
Theoretical weight: 29.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27540 (Residues: 84-345; Coverage: 33%)
Gene names: ARNT, BHLHE2
Sequence domains:
DNA (5'-D(*GP*GP*TP*CP*AP*CP*GP*CP*AP*AP*CP*C)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
DNA (5'-D(*GP*GP*TP*TP*GP*CP*GP*TP*GP*AP*CP*C)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 12 nucleotides
Theoretical weight: 3.69 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6522
Unit cell:
a: 91.321Å b: 91.321Å c: 464.914Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.294 0.292 0.333
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli