PDBe 5nhu

X-ray diffraction
1.45Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
AGAP008004-PA Chains: I, J, K
Molecule details ›
Chains: I, J, K
Length: 82 amino acids
Theoretical weight: 8.94 KDa
Source organism: Anopheles gambiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7Q3R9 (Residues: 22-103; Coverage: 100%)
Gene names: 1277947, AgaP_AGAP008004

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Unit cell:
a: 110.71Å b: 78.64Å c: 136.109Å
α: 90° β: 101.84° γ: 90°
R-values:
R R work R free
0.18 0.179 0.203
Expression system: Escherichia coli BL21