PDBe 5n10

X-ray diffraction
1.6Å resolution

Cucurbit[8]uril and 14-3-3 based binary bivalent supramolecular-protein assembly platform

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Estrogen receptor Chains: C, D, F
Molecule details ›
Chains: C, D, F
Length: 15 amino acids
Theoretical weight: 1.53 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P03372 (Residues: 584-595; Coverage: 2%)
Gene names: ESR, ESR1, NR3A1
14-3-3 protein beta/alpha Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31946 (Residues: 1-246; Coverage: 100%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
14-3-3 protein beta/alpha Chain: B
Molecule details ›
Chain: B
Length: 249 amino acids
Theoretical weight: 28.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31946 (Residues: 1-246; Coverage: 100%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 133.807Å b: 57.571Å c: 93.45Å
α: 90° β: 98.64° γ: 90°
R-values:
R R work R free
0.17 0.17 0.194
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)