5mbv

Electron Microscopy
3.8Å resolution

Cryo-EM structure of Lambda Phage protein GamS bound to RecBCD.

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
RecBCD enzyme subunit RecB Chain: B
Molecule details ›
Chain: B
Length: 1181 amino acids
Theoretical weight: 134.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08394 (Residues: 1-1180; Coverage: 100%)
Gene names: JW2788, b2820, ior, recB, rorA
Sequence domains:
Structure domains:
RecBCD enzyme subunit RecC Chain: C
Molecule details ›
Chain: C
Length: 1122 amino acids
Theoretical weight: 128.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07648 (Residues: 1-1122; Coverage: 100%)
Gene names: JW2790, b2822, recC
Sequence domains:
Structure domains:
RecBCD enzyme subunit RecD Chain: D
Molecule details ›
Chain: D
Length: 609 amino acids
Theoretical weight: 67.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04993 (Residues: 2-608; Coverage: 100%)
Gene names: JW2787, b2819, hopE, recD
Sequence domains:
Structure domains:
Host-nuclease inhibitor protein gam Chains: A, E
Molecule details ›
Chains: A, E
Length: 98 amino acids
Theoretical weight: 11.66 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03702 (Residues: 41-138; Coverage: 71%)
Gene names: gam, gamma
Sequence domains: Host-nuclease inhibitor protein Gam
Structure domains: Host-nuclease inhibitor protein Gam

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-3460
Expression system: Escherichia coli BL21(DE3)