5m5b

X-ray diffraction
2.01Å resolution

Crystal structure of Zika virus NS5 methyltransferase

Released:

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase NS5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 30.36 KDa
Source organism: Zika virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A024B7W1 (Residues: 2525-2786; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 37.55Å b: 64.14Å c: 72.04Å
α: 113.05° β: 97.78° γ: 92°
R-values:
R R work R free
0.162 0.16 0.191
Expression system: Escherichia coli BL21